10x 蛋白电转移缓冲液(转膜液/电转液)

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  • 16.67/

北京兰博利德商贸有限公司
15711203280 01060608020
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  • Brand  Lablead/兰博利德
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Last Updated 2025-08-12 18:42

10x 蛋白电转移缓冲液(转膜液/电转液)

产品货号:T3011

储存条件:室温保存

使用说明

稀释后用于蛋白电转移:

l 稀释为1×转移缓冲液含20%甲醇后使用。大部分蛋白质电转移,无甲醇不明显影响转移效果。

注意:本品不含SDS

1、由于蛋白分布的原因,如分布于细胞膜的蛋白疏水性很强,没有SDS存在目的蛋白易沉积在凝胶中。

2、高分子蛋白也有可能出现溶解性不足的可能,特别是处于凝胶上样缓冲液的变性条件和含甲醇的转膜缓冲液的情况下更是如此。

转膜缓冲液里SDS浓度应该不超过0.1%,并保证有足够的平衡时间从凝胶里去除SDS。

Company Name 北京兰博利德商贸有限公司
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