一步法SYBR FAST qPCR试剂5ml,500x20μ

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北京启衡星生物科技有限公司
15801217100 010-62149251
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  • Brand  KAPA Biosystems
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  • Main Business:核酸扩增,核酸提取,Qubit核酸浓度定量,文库定量,核糖体(rRNA)去除,诊断原料,分子检测试剂CDMO
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Last Updated 2025-08-12 18:44

一步法SYBR FAST qPCR试剂5ml,500x20μ

一步法SYBR FAST qPCR试剂  含反转录和定量试剂 

1、产品描述

KAPA one -Step qRT-PCR是一款灵敏、方便的定量试剂盒。试剂盒包含KAPA SYBR® FAST qPCR Master Mix (2x )、KAPA RT Mix ( 50x )和dUTP。其中KAPA RT Mix ( 50x )包括野生型M-MuLV转录酶、RNase 抑制剂。操作者只需加入引物和RNA即可,一步完成反转录和qPCR两个过程,方便简单快速。适用各种荧光定量PCR 仪,如罗氏LightCycler480、ABI Prism、伯乐iCycler等。

2、主要应用

●基因表达分析;●RNA拷贝数检测(病毒及病菌检测);●低拷贝数基因检测;●基因表达验证;
●基因敲除验证;●RNAi和miRNA研究。

3、主要特点

 本试剂盒包含了KAPASYBR® FAST产品的所有优点,此外,客户反馈其优于市场同类产品。

4、主要优势

●更快速;●Ct值更早出现;●对低拷贝数更敏感;●操作简单。

1)设定反应体系

对比测试结果

上面2幅图片显示,用KAPA SYBR® FAST one-step qRT-PCR与其他公司产品对比检测10倍稀释人RNA(100ng-100pg/reaction)。通过 KAPA SYBR® FAST one -step qRT-PCR试剂盒(红色曲线)与Competitor 1(图A)及Competitor 2 (图B)的对比,可看出KAPA产品具较强的信号及较早的Ct值。

5、产品信息

货号 产品 规格 说明书
KK4650 通用型一步法SYBR Fast 1mL,100x20μl

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KK4651 通用型一步法SYBR Fast 5ml,500x20μl
KK4652 通用型一步法SYBR Fast 10ml,1000x20μl
KK4660 ABIPdsmTM—步法SYBR快速定量 1ml,100x20μl

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KK4661 ABIPrismTM一步法SYBR快速定量 5ml,500x20μl
KK4662 ABIPrfsmTM—步法SYBR快速定量 10ml,1000x20μl
KK4670 Bio-RadiCyclerTM一步法SYBR快速定量 1ml,100x20μl

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KK4671 Bio-RadiCyclerTM一步法SYBR快速定量 5ml,500x20μl
KK4672 Bio-RadiCyclerTM一步法SYBR快速 10ml,1000x20μl
KK4680 RocheLightCycler 480—步法SYBR快速定量 1ml,100x20μl

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KK4681 RocheLightCycler 480—步法SYBR快速定量 5ml,500x 20μl
KK4682 RocheLightCycler 480—步法SYBR快速定量 10ml,1000x20μl

Company Name 北京启衡星生物科技有限公司
Location
Business Scope 核酸扩增,核酸提取,Qubit核酸浓度定量,文库定量,核糖体(rRNA)去除,诊断原料,分子检测试剂CDMO
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