Dispase酶Ⅱ（中性蛋白酶）

Dispase II (neutral protease）, Dispase酶Ⅱ（中性蛋白酶）
4942078001,5 x 1 g non-sterile

EC 3.4.24.4
Enzyme activity: ≥0.8 U/mg (37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg 2+, other heavy metals. Dispase is not inhibited by serum.
Specificity: Dispase is a nonspecific metalloprotease.
Working concentration: 0.6 to 2.4 U/ml

Background Information

One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute under assay conditions. A practical comparison of Roche units of Dispase ® II with those cited in the Japanese literature (where dispase concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.

Contents

5 vials with 1 g each; non-sterile lyophilizate

Preparation of Stock and Working Solution:
Dissolve the non-sterile lyophilized enzyme in Hepes-buffered saline (50 mM Hepes/KOH pH 7.4, 150 mM NaCl) (10 mg/ml). Dilute further with the culture medium to be used for the isolated cells to a final concentration of 0.6 to 2.4 U/ml. Concentrations higher than 2.4 U/ml are not recommended. Sterilize through a 0.22 μm filter membrane.