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MinuteTM 质膜脂筏分离试剂盒
目录号：LR-042
描述：
脂筏是含有高水平胆固醇和鞘脂的小质膜结构域。脂筏被发现存在于质膜（PM）和一些内膜系统中，如线粒体相关膜（MAMS）和内质网等。脂筏参与许多细胞过程，如信号转导，膜转运和蛋白质分选。脂质修饰的蛋白质和一些跨膜蛋白质集中在脂筏中，而其他蛋白质被排除在外。脂筏还被发现与质膜上的Na+ / K+ ATP酶相关。传统的脂筏分离方法是从总膜结构中分离出脂筏，而不能分质膜或者细胞器膜上的脂筏。为了克服传统方法的缺点，我们开发了此款基于离心管柱技术的质膜脂筏分离试剂盒。首先较大的质膜囊泡被分离出来，然后使用含有非离子表面活性剂缓冲液处理，通过台式离心机即可离心分离质膜脂筏部分。不使用传统的匀浆机和超速离心机，1h左右即可高度富集质膜脂筏。
*如需分离总的脂筏，请使用 Minute TM 脂筏分离试剂盒 Cat#LR-039
应用：
提取的蛋白可用于SDS-PAGE ，ELISA ，IP，2D胶分析，质谱，酶活检测以及其他应用。
Protocol：
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