Language
Biotin-14-dCTP描述
Biotin-14-dCTP是一款已获专利的 dCTP类似物，在嘧啶基的N 4 –位通过一个14-原子连接头连接生物素。这种核苷酸能够在dATP，dGTP，和dTTP存在的条件下通过缺口平移有效地掺入DNA (1)；或者用末端脱氧核苷酸转移酶 (2) 或者随机引物合成 (3) 标记DNA。所提供的材料数量足够标记多达50 µg的 DNA。性能和质量测试：纯度由反相HPLC评估。
规格
| Label or Dye: | Biotin |
|---|---|
| Product Size: | 50 nmol |
| Concentration: | 0.4 mM |
| Labeling Method: | Direct Labeling |
| Shipping Condition: | Dry Ice |
内容及储存
Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.引用及参考文献
Comparison of detection and signal amplification methods for DNA microarrays. Vora GJ, Meador CE, Anderson GP, Taitt CR,Mol Cell Probes (2008) 22:294-300
Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase. Flickinger JL, Gebeyehu G, Buchman G, Haces A, Rashtchian A,
Nucleic Acids Res (1992) 20:2382-2382
Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA. Gebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L
Nucleic Acids Res (1987) 15:4513-4534
