Language
浙江一级代理 杭州昊鑫生物
样品中的DNA和蛋白也能相继以沉淀的方式还原。乙醇沉淀能析出中间层的DNA，在有机层中加入异丙醇能沉淀出蛋白。共纯化DNA对于样品间标准化RNA的产量十分有用。
无论是人、动物、植物还是细菌组织，该方法对少量的组织(50-100mg)和细胞(5×106)以及大量的组织(≥1g)和细胞(>107)均有较好的分离效果。TRIpure 试剂操作上的简单性允许同时处理多个的样品。所有的操作可以在一小时内完成。TRIpure抽提的总RNA能够避免DNA和蛋白的污染。故而能够作RNA印迹分析、斑点杂交、poly(A)+选择、体外翻译、RNA酶保护分析和分子克隆。如果是用于PCR，当两条引物位于单一外显子内时，建议用扩增级的DNase I来处理抽提的总RNA。
TRIpure 试剂能促进不同种属不同分子量大小的多种RNA的析出。例如，从大鼠肝脏抽提的RNA琼脂糖凝胶电泳并用溴化乙啶染色，可见许多介于7 kb和15 kb之间不连续的高分子量条带(mRNA和hnRNA成分)，两条优势核糖体~5 kb (28S)和~2 kb(18S)，低分子量RNA介于0.1和0.3 kb之间 (tRNA, 5S)。当抽提的RNA用TE稀释时其A260/A280比值≥1.8。
适用范围：
适用于各种动植物组织/细胞总RNA、DNA、蛋白的快速抽提
使用该产品发表的部分文章：
| 1. Effect and Molecular Mechanism of DHA on Improving Rats Learning and Memory Ability. Food Science, 2012, online 2. Differential expression of microRNAs in human keloids. Chinese Journal of Tissue Engineering Research, 2012, 16(50): 9370-9375. 3. Study of Multidrug-resistance Reversing of Curcumin and Verapamil on Human Gastric Carcinoma SGC7901/ADR Cells.Vol.34,No.2,Apr.2012,pp.322-325 4. Effect of Sanguisorba tannins on the expression of MGMT gene and protein in myelosuppressed mice. TUMOR Vol. 33, September 2013 5. Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126 6. Effects of Du Meridian Electroacupuncture on Growth Associated Protein- 43 Expression in Rats after Spinal Cord Injury. Chin J Rehabil Theory Pract, Jan. 2014, Vol. 20, No.1. 7. Mycobacterium Bovis Ornithine Carbamoyltransferase,MB1684, Induces Proinflammatory Cytokine Gene. Expression by Activating NF-kB in Macrophages. ORIGINAL RESEARCH ARTICLE. DNA AND CELL BIOLOGY. Volume 33, Number 5, 2014ª Mary Ann Liebert, Inc.Pp. 1–9. DOI: 10.1089/dna.2013.2026. 8. A Novel Method of Total RNA Extraction from Leaves of.China Biotechnology,2013,33(5):107-111 9. The Response to High Magneto-Gravitational Environment of Different Differentiating-Stage Osteoblasts. Chinese Journal of Cell Biology 2013, 35(7): 10. Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51 |
