EASYspin Plus细菌RNA快速提取试剂盒
浙江省一级代理:杭州昊鑫生物
产品介绍:
本公司推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术确保有效清除gDNA残留,得到的RNA不需要DNase消化,可直接用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶,然后裂解混合物通过一个基因组DNA清除柱,基因组DNA被清除而RNA穿透滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。
产品特点:
1.完全不使用有毒的苯酚,氯仿,beta巯基乙醇等试剂,也不需要乙醇沉淀等步骤。
2.快速,简捷,单个样品操作一般可在30分钟内完成。
3.独家研发成功基因组DNA清除柱技术确保有效清除gDNA残留,得到的RNA不需要DNase消化,可直接用于反转录PCR、荧光定量PCR等实验。
4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.0,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。
使用该产品发表的部分文章:
EASYspin 细菌RNA提取试剂盒发表文章
- Cloning and characterization of bagB and bagC, two co-transcribed genes involved in bagremycin biosynthesis in Streptomyces sp. Tü 4128. Ann Microbiol 2013,63(1), : 167–172
EASYspin Plus细菌RNA提取试剂盒发表文章
- Relative significances of pH and substrate starch level to roles of Streptococcus bovis S1 in rumen acidosis. AMB Express 2016, 6:80 DOI: 10.1186/s13568-016-0248-2
- Design of Ribosome Binding Sites in Streptomyces coelicolor.Current Proteomics 2017, 14(4): 287-292
- Antibacterial activity of gallic acid against Shigella flexneri and its effect on biofilm formation by repressing mdoH gene expression. Food Control 2018, 94: 147-154
- Study on the drug resistance of clinical strain of acinetobacter baumannii mediated by TEM-1 beta-lactamases and porin CarO. Tianjin Med J, 2018, 46(3): 246-250
- Contribution of the colicin receptor CirA to biofilm formation, antibotic resistance, and pathogenicity of Salmonella Enteritidis. J Basic Microbiol. 2019, 1–10. https://doi.org/10.1002/jobm.201900418
- FadR1, a pathway-specific activator of fidaxomicin biosynthesis in Actinoplanes deccanensis Yp-1. Applied Microbiology and Biotechnology 2019, https://doi.org/10.1007/s00253-019-09949-y

