Fop-Flash

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Fop-Flash,Plasmid Fop-Flash,Fop-Flash vector

 

Fop-Flash Information

Promoter: TK

Replicon: pUC ori

Terminator: SV40 poly (A) signal

Plasmid classification: mammalian cells, signal pathway reporting vectors

Prokaryotic resistance: ampicillin Amp

Cloned strain: Escherichia coli DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: mammalian cells

5'sequencing primers: primers designed according to sequence

3'sequencing primers: primers designed according to sequence

 

Fop-Flash Sequence

LOCUS       Exported                5529 bp ds-DNA     circular SYN 24-APR-2018 DEFINITION  synthetic circular DNA FEATURES             Location/Qualifiers      source          1..5529                      /organism="synthetic DNA construct"                      /mol_type="other DNA"      promoter        590..652                      /note="Mini-TK promoter"                      /note="minimal herpes simplex virus (HSV) thymidine kinase                       promoter"      CDS             681..2333                      /codon_start=1                      /gene="luc"                      /product="firefly luciferase"                      /note="luciferase"                      /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA                      HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP                      ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS                      MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA                      RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK                      IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY                      GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS                      GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL                      LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV                      FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"      intron          2492..2557                      /note="small t intron"                      /note="simian virus 40 (SV40) small t antigen intron"      CDS             2687..2707                      /codon_start=1                      /product="nuclear localization signal of SV40 large T                       antigen"                      /note="SV40 NLS"                      /translation="PKKKRKV"      polyA_signal    3132..3266                      /note="SV40 poly(A) signal"                      /note="SV40 polyadenylation signal"      rep_origin      complement(3710..4298)                      /direction=LEFT                      /note="ori"                      /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                       replication"      CDS             complement(4469..5329)                      /codon_start=1                      /gene="bla"                      /product="beta-lactamase"                      /note="AmpR"                      /note="confers resistance to ampicillin, carbenicillin, and                      related antibiotics"                      /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                      ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS                      PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                      EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                      LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                      LIKHW"      promoter        complement(5330..5434)                      /gene="bla"                      /note="AmpR promoter" ORIGIN         1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca        61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg       121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc       181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc       241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat       301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt       361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agggggtaag gccaaagggg       421 gtaaggccaa agggggcccc ctttggcctt accccctttg gccttacccc ctttggcctt       481 acctgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc ttgtcattgg       541 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta       601 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca       661 acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat       721 tctatcctct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg       781 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg       841 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata       901 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg       961 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat      1021 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc      1081 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt      1141 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc      1201 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac      1261 tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa      1321 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg      1381 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg      1441 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt      1501 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat      1561 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt      1621 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc      1681 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg      1741 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg      1801 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag      1861 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca      1921 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca      1981 tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat      2041 tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg      2101 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga      2161 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg      2221 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa      2281 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa      2341 ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc caccgcggtg      2401 gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga      2461 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg      2521 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga      2581 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc      2641 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag      2701 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt      2761 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgc  <