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GenJet™ DNA In Vitro Tranfection Reagent for MDCK cell is pre-optimized for transfecting MDCK cells. MDCK cell line is derived from the kidney of an apparently normal, adult, female cocker spaniel; used in numerous viral studies; susceptible to vesicular stomatitis, vaccinia, and Coxsackie viruses, and certain reoviruses and adenoviruses.
The "Shaved Cell Transfection" protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal transfection conditions for maximal transfection efficiency on MDCK cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.
| Summary of Optimal Shaved CellTransfection Conditions: Cell culture medium Confluency of cell on the day of shaving Cell density for plating GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Incubation time of shaved cells with transfection complex Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) | DMEM with 4.5 g/L glucose, 10% FBS 95~100% 1.3x10^5 cells per square centimeter 4:1 Serum-free DMEM with 4.5 g/L glucose 12 minutes at RT 20 minutes at 37 degree 48 hours EGFP pEGFP-N3 (CMV promoter) 70% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on MDCK Cells. MDCK cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
