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| 试剂名称 | 规格 |
| PFA | 80 ml (4%) |
| PBS | 10 ml (10×) |
| Tween-20 | 50 μl |
| Protein K | 10 μl (10 μg/μl) |
| Formamide | 15 ml |
| SSC | 6 ml (20×) |
| yeast RNA | 100 μl (50 mg/ml) |
| Heparin sodium salt | 10 μl (50 mg/ml) |
| Customized gene probe | YSY BIOTECH www.njfish.cn |
| MAB | 200 ml |
| Blocking Reagent | 4 ml (10%) |
| sheep serum | 2 ml |
| Anti-Digoxigenin-AP,Fab fragments | 5 μl |
| (-)Tetramisole hydrochloride | 60 μl (0.2 mg/μl) |
| NBT/BCIP Stock Solution | 100 μl |
| equilibrium solution | 10 ml |
