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Hieff Unicon® Hotstart Direct

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  • 800.28/

翌圣生物科技(上海)股份有限公司
13916312745 400-6111-883
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  • Brand  翌圣生物(Yeasen)货号10718ES01
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  • Main Business:分子生物酶,科研试剂及试剂盒,
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Brand 翌圣生物(Yeasen)货号10718ES01
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Last Updated 2025-08-12 18:58

Hieff Unicon® Hotstart Direct

产品信息
产品名称 产品编号 规格 价格(元)
Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 50 U/μL 10718ES01 100 μL 5762.00
Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 50 U/μL 10718ES94 1 mL 34962.00
Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 50 U/μL 10718ES95 25 mL 询价
Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 50 U/μL 10718ES96 100 mL 询价

产品描述
Hieff Unicon® Hotstart Direct Taq DNA Polymerase是一种耐受血液及其它抑制物的热启动DNA聚合酶。本产品采用抗体封闭,具备良好的扩增灵敏度和特异性。本品在预变性温度下加热30 sec封闭抗体即可完全失活,释放出 DNA 聚合酶活性。使用该热启动Taq酶可以有效抑制引物非特异性退火导致的扩增。


产品组分
组分编号 组分名称 10718ES01
(5 KU)
10718ES94
( 50 KU)
10718ES95
(1250 KU)
10718ES96
(5000 KU)
10718-A Hotstart D-Taq (50 U/μL) 100 μL 1 mL 25 mL 100 mL

运输与保存方式
冰袋运输。-20℃储存,有效期1年。

反应体系
组分 体积 (μL) 终浓度
2×Buffera 25
Primer/Probe mixb X 0.1 μM-0.5 μM
Hotstart D-Taq (50 U/μL) 0.12 0.12 U/μL
模板DNAc X 0.1-100 ng
无菌超纯水 up to 50 -
【注】 a)根据具体的实验应用,需自备相应的反应Buffer。如需要基础反应Buffer (10×),推荐货号11373ES。
b/d)上表中DNA量和引物浓度均为推荐浓度,可根据具体实验情况调整最适浓度。
c)请根据具体的实验应用,调整酶加入量。

参考扩增程序(两步法)
循环步骤 温度 时间 循环数
预变性 95℃ 5 min 1
变性 95℃ 15 sec 45
退火/延伸 60℃a 30 secb
【注】a)扩增反应:扩增反应温度根据设计的引物Tm值进行调整。
b)荧光信号采集:不同的qPCR仪器所需的荧光信号采集时间不同,请根据最短时间限制进行设置。

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HB200522
 
Company Name 翌圣生物科技(上海)股份有限公司
Location
Business Scope 分子生物酶,科研试剂及试剂盒,
Contact Information
Medicine Platform
 
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