转染试剂X-tremeGENE™ siRNA
(目录价¥14459.14元/瓶)
13611631389上海睿安生物 一般描述:
转染操作可将小干扰RNA(siRNA)直接递送至细胞中。X-tremeGENE siRNA转染试剂是一种脂质体转染试剂，为向动物细胞中递送siRNA提供了一种方便、可靠和高效的途径，便于用户进行基因敲除后的细胞及功能学研究。这一创新型试剂能够与siRNA以及siRNA和质粒的混合物(共转染的情况下)形成复合物，并有效递送这些核酸进入细胞，以介导基因沉默。只需简单的几步就可完成转染:首先将转染试剂与稀释过的siRNA混合并孵育，之后将复合物直接加入细胞培养物中。由于X-tremeGENE siRNA转染试剂在有无血清的条件下均工作良好，且经论证无明显细胞毒性，因此无需更换培养基。本品不含任何动物源性成份。
组分:本试剂经0.2μm滤膜过滤，聚丙烯小管包装。
应用:X-tremeGENE siRNA转染试剂能够有效将短干扰RNA(siRNAs)转入许多种常用细胞，包括HeLa，NIH 3T3，HEK-293，CHO-K1和COS-7和一些难转染细胞系，比如HT29，一种人腺癌细胞系。
特点和优势:
1、在多种不同类型细胞中基因敲除效率超过90%；
2、高灵活应用， 同一试剂可用于siRNA和质粒共转染的基因沉默实验；
3、细胞毒性低，结果相关性好，确保所观察到的细胞效应是由转染的siRNA而非转染过程本身介导；
4、兼容含血清或不含血清的培养基，转染前后均无需换液(如无血清培养基)。
X-tremeGENE siRNA转染试剂是一款包含脂质体及其他组份的专利混合试剂，无动物源成分。
质量:活性分析：将X-tremeGENE siRNA转染试剂(1-2.5μl)与特异性靶向HPRT看家基因的siRNA(0.1-0.35μg)相互混合。在含10%胎牛血清(FBS)的条件下，将此混合物用于转染HEK-293细胞(生长密度为30-50%的单层细胞)。转染后通过LightCycler实时荧光定量PCR来检测细胞中HPRT mRNA水平的降低情况。测得的mRNA数据显示，观察到的敲除效率通常处于70-95%。
细胞毒性分析:HEK-293细胞暴露于siRNA/X-tremeGENE siRNA试剂混合物中72小时，未对含有血清的培养基进行换液。之后通过WST-1细胞增殖试剂(Roche)分析细胞，以测试细胞毒性。
其他说明:仅用于生命科学研究。不可用于诊断。法律信息:X-tremeGENE is a trademark of Roche。
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