Alkaline Phosphatase Live Stai

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  • 471.94/

上海觅拓生物科技有限公司
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  • Brand  Invitrogen™
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  • Main Business:细胞,载体,试剂,技术服务,实验室耗材
The information on this page regarding the "Alkaline Phosphatase Live Stai" product is provided by 上海觅拓生物科技有限公司. If you would like to know more about the price, model, and manufacturer of "Alkaline Phosphatase Live Stai", please contact the supplier or leave a message.
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Last Updated 2025-08-12 19:13

Alkaline Phosphatase Live Stai

描述

碱性磷酸酶活性染料是一种干细胞成像产品,用户可利用其对多能性干细胞 (PSC) 进行鉴别染色。该染料是碱性磷酸酶 (AP) 的细胞可渗透荧光底物,对细胞无毒性,两个小时后即可扩散出去。碱性磷酸酶活性染料包括50 µL的500X荧光染料 (DMSO溶解),足够完成两块24孔板、四块6 cm培养皿或两块10 cm培养皿的染色。

• 识别信号高于背景的多能干细胞

• 率先使用能维持细胞活性的碱性磷酸酶染料

• PSC染色一般只需20分钟甚至更短时间

碱性磷酸酶多能性干细胞检测方法

AP是一种PSC表型标志物,包括未分化的胚胎干细胞 (ESC)、诱导多能性干细胞 (iPSC) 和胚胎生殖细胞 (EGC)。AP在大多数细胞类型中均有表达,但其在PSC内的表达水平明显升高。因此AP染色已应用于PSC的鉴别染色,采用该方法可轻松将PSC与用作饲养细胞的小鼠胚胎成纤维细胞 (MEF) 及重编程实验中常用的亲代成纤维细胞区分开来。

无需牺牲宝贵的集落,即可实现多能性筛选

碱性磷酸酶活性染料可维持干细胞的活性,因此适用于在重编程流程的早期筛选集落。它还可在较晚期作为阴性选择工具用于鉴别未分化的细胞。

快速细胞染色

碱性磷酸酶活性染料采用了简单、非永久性的细胞活性实验方案,用于您的实验中PSC的鉴别。只需用培养基稀释染料,加入细胞中,轻轻冲洗,即可用于荧光成像。

仅供研究使用。不得用于诊断。

规格

Color: Green
For Use With (Equipment): Floid® Cell Imaging System, Fluorescence Microscope
Sub-Cellular Localization: Cell Membranes & Lipids
Detection Method: Fluorescent
Form: Liquid
Label or Dye: FITC (Fluorescein)
Product Size: 50 µL
Quantity: 1 tube(s)
Sample Type (Specific): Live Cells
Shipping Condition: Dry Ice

内容及储存

1个棕色瓶,置于 -20°C保存
Company Name 上海觅拓生物科技有限公司
Location
Business Scope 细胞,载体,试剂,技术服务,实验室耗材
Contact Information
Medicine Platform
 
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