KAPA 2G Robust DNA聚合酶及Ready Mi

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北京启衡星生物科技有限公司
15801217100 010-62149251
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  • Brand  KAPA Biosystems
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  • Main Business:核酸扩增,核酸提取,Qubit核酸浓度定量,文库定量,核糖体(rRNA)去除,诊断原料,分子检测试剂CDMO
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Last Updated 2025-08-12 19:19

KAPA 2G Robust DNA聚合酶及Ready Mi

KAPA 2G Robust DNA聚合酶及Ready Mix 复杂PCR简单化

1、主要特点

1)扩增各种困难、复杂模板(GC或AT含量高)效果良好;2)对抑制因子不敏感;

3)单位酶的产量和灵敏度高;4)针对复杂模板,配备特定的增强剂。

2、应用范围

1)同样可用于普通的PCR反应;2)AT和GC碱基含量高和扩增;

3)模板含常见的PCR反应抑制因子,例如,盐离子、尿素、SDS、乙醇和EDTA等;

4)石蜡油包被样品;5)粪便样本;6)菌落PCR。

3、优异表现

4、PCR反应

1)推荐反应体系

注意:各种Buffer使用推荐

1)Buffer A:推荐扩增CG含量<65%的模板;

2)Buffer B:推荐扩增含杂质、阴离子抑制剂和模板,其成分与Buffer A差异很大;

3)GC Buffer:推荐扩增GC含量高的模板,或因二级结构难以扩增的模板;

4)Enhancer 1 :可提高反应效率和特异性,但不是适合所有模板;

5)PCR扩增中遇到困难,首先测试Buffer A或Buffer B的效果,GC含量高时可以尝试使用GC Buffer,GC含量不高时也可尝试GC Buffer,但GC Buffer不能与Enhancer1同时使用。对于特殊复杂的模板,推荐一下组合①1×GC Buffer+4%DMSO;②1×Buffer A+5%DMSO+1×KAPA enhancer 1。

2)推荐反应参数:

5、产品信息

货号产品规格
KK50232G Robust 酶100 units
KK50242G Robust 酶250 units
KK50042G Robust 酶(含dDNTs)100 units
KK50052G Robust 酶(含dDNTs)250 units
KK55222G Robust 热启动酶100 units
KK55152G Robust 热启动酶250 units
KK55172G Robust 热启动酶500 units
KK55252G Robust 热启动酶2500 units
KK55322G Robust 热启动酶(含dDNTs)100 units
KK55162G Robust 热启动酶(含dDNTs)250 units
KK55182G Robust 热启动酶(含dDNTs)500 units
KK57012G Robust 热启动酶ReadyMix100×25μl
KK57022G Robust 热启动酶ReadyMix500×25μl

 

 

 

 

Company Name 北京启衡星生物科技有限公司
Location
Business Scope 核酸扩增,核酸提取,Qubit核酸浓度定量,文库定量,核糖体(rRNA)去除,诊断原料,分子检测试剂CDMO
Contact Information
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