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操作步骤： (依据上表以 24 孔板为例，DNA 转染） 1、准备待转染细胞：按照贴壁细胞 5×104/孔的密度接种于 24 孔板中，37 ℃培养过夜。 2、转染前 30 分钟，将待转细胞更换新鲜无血清培养基。 3、准备 FectinMore/DNA 复合物：将 0.5μg DNA 溶于 25μL 无血清培养基中混匀，再将 1.5 μL FectinMore 加入另外 25 μL 无血清培养基中混匀，各自于室温反应 5 分钟后，再将两者混匀后得到的 50μL 复合物室温孵育 15 分钟。 4、转染：将上述 FectinMore/DNA 复合物加入每孔细胞（含无血清细胞培养基 450 μL），复合物占总体积的 1/10，轻柔摇匀。37 ℃孵育 24-48 小时。必要时，转染 6 小时后可更换新鲜有血清培养基。
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