Biolegend货号349509，APC anti-human CD81(TAPA-1)，(规格包装25次/盒)(目录价¥1525.00元/盒)13611631389上海睿安生物
Biolegend货号349509，APC anti-human CD81(TAPA-1)，(规格包装25次/盒)(目录价¥1525.00元/盒)13611631389上海睿安生物
DescriptionCD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.
roduct Details
Isotype Control
APC Mouse IgG1, κ Isotype Ctrl (FC)
Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human OCI-LY8 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser
Red Laser (633 nm)
Application Notes
Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.
Application References
(PubMed link indicates BioLegend citation)
Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP)
Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB)
Mochida K, et al. 2008. J. Virol. 13:6711.
Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
Product Citations
Abe I, et al. 2022. Dev Cell. 57:2623. PubMed
Kocak P, et al. 2023. Aesthet Surg J Open Forum. 5:ojad039. PubMed
Ragni E, et al. 2021. Cells. 10: . PubMed
Ragni E, et al. 2022. Biomolecules. 12:. PubMed
Boruchowicz H, et al. 2020. J Virol. :94. PubMed
Ragni E, et al. 2022. Pharmaceutics. 14:. PubMed
Ragni E, et al. 2020. Stem Cell Res Ther. 165:11. PubMed
Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
Angeloni N, et al. 2016. Sci Rep. 6:22915. PubMed
Zimmerman B et al. 2016. Cell. 167(4):1041-1051 . PubMed
Najm FJ, et al. 2018. Nat Biotechnol. 36:179. PubMed
Samelson AJ, et al. 2022. Nat Cell Biol. 24:24. PubMed
See More
RRID
AB_2564020 (BioLegend Cat. No. 349509)
AB_2564021 (BioLegend Cat. No. 349510)
