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无内毒素质粒小提试剂盒(离心柱法)

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  • 33.33/

盈卡科学器材(上海)有限公司
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  • Brand  GENECARD
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Last Updated 2025-08-12 19:56

无内毒素质粒小提试剂盒(离心柱法)

该试剂盒使用 SDS 和碱裂解方法,以及特殊的缓冲液 P4 和过滤柱 GS,并结合玻璃纤维素膜特异性吸附方法对溶液中的 DNA。能有效去除内毒素和蛋白质等杂质,适用于从 5-15 ml 细菌培养物中提取质粒 DNA。整个提取过程只需一个小时,方便快捷。纯化的质粒 DNA 可用于各种常规作,包括一些常规传代细胞的转染、酶切、PCR、测序、连接、转化、文库筛选、体外翻译等。该试剂盒已针对缓冲液 P2 进行了优化,以避免在细菌种群极低或细菌溶液过度老化时可能出现单链质粒 DNA。同时,Buffer P4 已经过优化,有效避免了 SDS 残留导致酶消化不完全或弥漫性以及转染效率低下。

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