HZB800001_pGEX-4T-1

产品名称	pGEX-4T-1
别称	pGEX4T1；pGEX4T-1
特征特性	宿主:大肠杆菌,片段类型:ORF,片段物种:空载体,原核抗性:Amp,启动子:Tac,复制子:pBR322,拷贝数:低,感受态:DH5a,温度:37度,正向引物:pGEX-5':GGGCTGGCAAGCCACGTTTGGTG,反向引物:pGEX-3':CCGGGAGCTGCATGTGTCAGAGG,诱导方式:IPTG
主要用途	蛋白表达
使用方法	干粉质粒：①收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒，室温放置1min；②从-80°C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；③取2μl质粒加至100μl感受态中，冰浴30min;④42°C热激90s，再冰浴2min;⑤加入900μl无抗的LB液体培养基，180rpm震荡培养45min;⑥6000rpm离心5min，仅留100μl上清混匀菌体沉淀；⑦混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；⑧将平板正向培养1h，再倒置培养12h～16h;⑨挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12h~16h，根据实验需要提取质粒。 注意：如果第二天转化平板长的过多，请将质粒按比例稀释后再转化。
规格	干粉(现货)，大提液体(一周)
保存说明	①干粉质粒：90天，请尽快转化。保存温度：一般在-20°C，置于-80°C保存时间更长。②液体质粒：存于-20℃，保质期90天。③滤纸质粒：存于-20度，保质期90天，请务必先转化提质粒后使用。

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HZB810727_pSuper-Puro-STRN-shRNA1(human)

HZB810728_pSuper-Puro-STK25-shRNA3(human)

HZB810729_pSuper-Puro-HK2-shRNA2(human)

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HZB810737_pCAG-GBP1-10gly-lexADBD

HZB810738_pSuper-Puro-STK25-shRNA1(human)

HZB810739_pSuper-Puro-PARPBP-shRNA3(human)

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HZB810741_pSuper-Puro-HK2-shRNA3(human)

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HZB810753_Tet-pLKO.Puro_shGFP

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HZB810755_pOTB7-NAGLU(human)(1同义突变1点突变)

HZB810756_pOTB7-SNX8(human)(2同义突变)

HZB810757_pOTB7-BCAR1-2(human)(2点突变1同义突变)

HZB810758_pOTB7-CYSRT1(human)(1点突变)

HZB810759_pMA2775

HZB810760_pOTB7-ARTN-2(human)

HZB810761_pOTB7-HIF3A(human)(2同义突变1点突变)

HZB810762_pOTB7-TMEM134(human)(875-1084缺失)

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HZB810764_pOTB7-LRRN4(human)(1点突变1同义突变)

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HZB810767_pOTB7-CTDNEP1(human)(1同义突变)

HZB810768_pCR4-TOPO-RSL1D1(human)(1同义突变1点突变)

HZB810769_pPCR-Script Amp SK(+)-CD200R1(human)(199-267缺失)

HZB810770_pCY 3010-07

HZB810771_pENTR223-Gpr158-m

HZB810772_pOTB7-SEPHS2(human)

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HZB810774_pOTB7-GLO1(human)

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HZB810778_pCMV-TSEN2(human)-3×FLAG-Neo

HZB810779_pOTB7-YY1AP1-3 (human)

HZB810780_pOTB7-BAP1(1同义突变)(human)

HZB810781_pLVX-mCherry-N1

HZB810782_pOTB7-HOXD3(1同义突变)(human)

HZB810783_pEnCMV-TNFRSF9(human)-3×FLAG-WPRE-SV40-Neo

HZB810784_pOTB7-TCP1(human)

HZB810785_pOTB7-VAC14(human)

HZB810786_pOTB7-OSBPL10(human)

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HZB810790_pOTB7-NIPSNAP1-2(human)

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HZB810827_pOTB7-UNC45A(human)(截短1927-2790bp)

HZB810828_pOTB7-MCM7-2(human)(1同义突变)

HZB810829_pOTB7-DDX31(human)(1点突变截短223-2556bp)

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HZB810874_pCMV-SPORT6-LMNTD1(human)(1点突变)

HZB810875_pCMV-SPORT6-WDR76(human)(1点突变)

HZB810876_pCMV-SPORT6-APOA1BP(human)

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HZB810878_pCMV-SPORT6-SC5D(human)(1同义突变)

HZB810879_pCMV-SPORT6-NMUR1(human)

HZB810880_pENTR223-BLOC1S2

HZB810881_pCMV-SPORT6-SVOPL(human)(1同义突变)

HZB810882_pCMV-SPORT6-NCAPG2-2(human)

HZB810883_pCMV-SPORT6-EPHB3(human)

HZB810884_pCMV-SPORT6-CD53(human)

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HZB810889_pCMV-SPORT6.ccdb-HLA-DQB2(human)(4同义突变1点突变)

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HZB810892_pBlueScriptR-VSIG10(human)(1同义突变)

HZB810893_pBlueScriptR-LIN7B(human)

HZB810894_pBlueScriptR-RHPN2(human)(1点突变)

HZB810895_pBlueScriptR-ZNF639(human)(1同义突变)

HZB810896_pBlueScriptR-MAX(human)

HZB810897_pBlueScriptR-MAPKAPK2(human)(1同义突变)

HZB810898_pCMV-SPORT6-CARD14-2(human)

HZB810899_pCMV-SPORT6-SEPTIN10(human)

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HZB810904_pBlueScriptR-FIBCD1(human)(1点突变)

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HZB810913_pCDH-CMV-Rnd2

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HZB810915_pCMV-SPORT6.ccdb-CUTC(human)

HZB810916_pBlueScriptR-RRP1B(human)(2同义突变1点突变)

HZB810917_pCMV-SPORT6.ccdb-C1R(human)(4同义突变5点突变)

HZB810918_pBlueScriptR-SPANXB1(human)(1点突变)

HZB810919_pCMV-SPORT6.ccdb-CA6(human)

HZB810920_pBlueScriptR-ABCF1(human)(1点突变)

HZB810921_pBlueScriptR-PLPP5(human)

HZB810922_pCMV-SPORT6.ccdb-PUSL1(human)

HZB810923_pCMV-SPORT6-NOL8(human)

HZB810924_pLXSN-TNF

HZB810925_pCMV-SPORT6.ccdb-CBLC(human)

HZB810926_pCMV-SPORT6.ccdb-TMEM44(human)

HZB810927_pBlueScriptR-RDM1(human)(1点突变)

HZB810928_pBlueScriptR-BRF1(human)(1同义突变)

HZB810929_pBlueScriptR-AGFG1(human)(2同义突变)

HZB810930_pBlueScriptR-ZDHHC19(human)

HZB810931_pEnCMV-ZDHHC13(human)(2同义突变)-V5-6×His-SV40-Neo

HZB810932_pBlueScriptR-POLM(human)

HZB810933_pCMV-SPORT6-CYP2U1(human)

HZB810934_pBlueScriptR-SWT1(human)(3同义突变4点突变)

HZB810935_pCDNA5FRT-FLAG-OGT1

HZB810936_pBlueScriptR-RGS22(human)(1点突变1同义突变)

HZB810937_pBlueScriptR-ECT2(human)

HZB810938_pBlueScriptR-ZNF165-2(human)(1同义突变)

HZB810939_pBlueScriptR-GRID1(human)

HZB810940_pBlueScriptR-CKAP2L(human)(1点突变)

HZB810941_pBlueScriptR-VTA1-2(human)

HZB810942_pCMV-SPORT6.ccdb-THRSP(human)

HZB810943_pCMV-SPORT6.ccdb-BDKRB1(human)(1同义突变)

HZB810944_pBlueScriptR-C1orf115(human)

HZB810946_pSXneo 270(T2AG3)

HZB810947_pBlueScriptR-DNAJC5B(human)

HZB810948_pBlueScriptR-SLC4A2(human)

HZB810949_pCMV-SPORT6-FTSJ3(human)

HZB810950_pCMV-SPORT6-CDC34(human)

HZB810951_pCMV-SPORT6.ccdb-ARSJ(human)

HZB810952_pCMV-SPORT6-TANK(human)

HZB810953_pCMV-SPORT6-YARS1(human)

HZB810954_pCMV-SPORT6-HOXC10(human)

HZB810955_pCMV-SPORT6-CNBP(human)(插入3碱基)

HZB810956_pCMV-SPORT6-SEH1L(human)

HZB810957_pUPRT-DHFR-D

HZB810958_pCMV-SPORT6.ccdb-LCE1B(human)

HZB810959_pCMV-SPORT6-PPWD1(human)(1同义突变)

HZB810960_pLVX-U6-TEX13C(human)-shRNA6-PGK-EGFP-E2A-Puro

HZB810961_pCMV-SPORT6-SLC35A5-2(human)

HZB810962_pCMV-SPORT6.ccdb-DNAJC10(human)

HZB810963_pCMV-SPORT6.ccdb-SERPINB8(human)

HZB810964_pCMV-SPORT6-FAM149B1(human)

HZB810965_pCMV-SPORT6-QTRT1(human)

HZB810966_pCMV-SPORT6-CCSAP(human)

HZB810967_pCMV-SPORT6-TRAPPC2(human)

HZB810968_pEX18TC

HZB810969_pCMV-SPORT6.ccdb-CALD1(human)

HZB810970_pCMV-SPORT6-ANGEL2(human)

HZB810971_pCMV-SPORT6-KCMF1(human)

HZB810972_pCMV-SPORT6-USP34(human)

HZB810973_pCR4-TOPO-SYN3(human)

HZB810974_pCR4-TOPO-LIF(human)

HZB810975_pCR4-TOPO-LANCL3(human)

HZB810976_pCR4-TOPO-TBX19(human)

HZB810977_pCR4-TOPO-PIN4(human)

HZB810978_pCR4-TOPO-WDPCP(human)

HZB810979_pC009 LshCas13a locus with targeting spacer

HZB810980_pCR4-TOPO-ZNF141(human)

HZB810981_pCR4-TOPO-BLM(human)(3同义突变)

HZB810982_pCR4-TOPO-PINX1(human)

HZB810983_pCR4-TOPO-EIF3D(human)

HZB810984_pCR4-TOPO-TM4SF5(human)

HZB810985_pCR4-TOPO-HIST1H4F(human)

HZB810986_pCR4-TOPO-CBLN1(human)

HZB810987_pCR4-TOPO-LILRA1(human)

HZB810988_pCR4-TOPO-HS3ST2(human)

HZB810989_pCR4-TOPO-H4C6(human)

HZB810990_pC011 LwCas13a locus with targeting spacer

HZB810991_pCR4-TOPO-PDZRN4(human)(1点插入)

HZB810992_pCR4-TOPO-SLC6A14(human)

HZB810993_pCR4-TOPO-CLEC4G(human)

HZB810994_pCR4-TOPO-UBXN2A(human)

HZB810995_pCR4-TOPO-SLC28A2(human)(1同义突变)

HZB810996_pCR4-TOPO-SERTAD2(human)

HZB810997_pDNR-LIB-ATP5MG(human)

HZB810998_pOTB7-MICAL1(human)

HZB810999_pOTB7-CAPN10(1同义突变1点突变)(human)

HZB811000_pDNR-LIB-CT83(human)

HZB811001_pEF1α-MCS-IRES-mCherry

HZB811002_pDNR-LIB-RPL32(human)

HZB811003_pDNR-LIB-CCL4L1(2点突变)(human)

HZB811004_pOTB7-STARD10(human)

HZB811005_pOTB7-DOCK5(human)

HZB811006_pOTB7-RECQL4(human)

HZB811007_pOTB7-UBQLN1(human)

HZB811008_pDNR-LIB-SLC2A12(1同义突变)(human)

HZB811009_pDNR-LIB-CLDN2(human)

HZB811010_pDNR-LIB-SPANXN3(1同义突变1点突变)(human)

HZB811011_pDNR-LIB-TRIAP1(human)

HZB811012_pmVenus-C1

HZB811013_pDNR-LIB-CFHR3(human)

HZB811014_pOTB7-CCNC(1同义突变)(human)

HZB811015_pDNR-LIB-CCDC58(human)

HZB811016_pDNR-LIB-S100A12(human)

HZB811017_pDNR-LIB-XCL1(human)

HZB811018_pOTB7-INT4(human)

HZB811019_pDNR-LIB-DEFB129(1同义突变)(human)

HZB811020_pDNR-LIB-RPS27(1点突变)(human)

HZB811021_pDNR-LIB-HGD(1点突变)(human)

HZB811022_pOTB7-NGLY1(1点突变)(human)

HZB811023_pEBFP2-N1

HZB811024_pOTB7-FUZ(第894个碱基缺失)(human)

HZB811025_pDNR-LIB-TRA@(human)

HZB811026_pDNR-LIB-TRA@(human)

HZB811027_pOTB7-SLC10A4(human)

HZB811028_pOTB7-SAMD11(1点突变1同义突变)(human)

HZB811029_pDNR-LIB-TRA@(human)

HZB811030_pDNR-LIB-NDUFB4(human)

HZB811031_pDNR-LIB-CRTAM(3点突变)(human)

HZB811032_pOTB7-FAM58A(human)

HZB811033_pOTB7-PDPK1(human)

HZB811034_pU6-Decoy

HZB811035_pDNR-LIB-RBPJ(1同义突变)(human)

HZB811036_pDNR-LIB-ABHD13(human)

HZB811037_pDNR-LIB-DLGAP1(human)

HZB811038_pcDNA3 AIF 1-90 mCherry FRB

HZB811039_pEnCMV-PIGX(human)-3×FLAG

HZB811040_pEnCMV-PRNP(mouse)-38aa-EGFP-39aa

HZB811041_pLVX-Puro-Notch(mouse)截短

HZB811042_pCDNA3.1-3×Myc-EEF1A1(human)

HZB811043_pCDNA3.1-3×Myc-EEF1A2(human)

HZB811044_pCDNA3.1-3×Myc-EEF1G(human)

HZB811045_MSP2135

HZB811046_pCDNA3.1-3×Myc-KRT1(human)

HZB811047_pCDNA3.1-3×Myc-KRT2(human)

HZB811048_pCDNA3.1-3×Myc-KRT5(human)(3同义突变)

HZB811049_pCold-GST-NUMB(human)

HZB811050_pEnCMV-IL13RA1(human)-3×FLAG

HZB811051_pCDNA3.1-3×Myc-KRT6A(human)

HZB811052_pLVX-NUMB(human)-3×FLAG-Puro

HZB811053_pCAMBIA1300-35S-NES-mCherry

HZB811054_pCAMBIA1381-AtK9I9-GUS

HZB811055_pCAMBIA1381-AtNUDT5-GUS

HZB811056_MSP977

HZB811057_pCAMBIA1381-AtCBF1-GUS

HZB811058_pCAGGS-MCS

HZB811059_pEnCMV-SLC2A1(mouse)-3×FLAG

HZB811060_pCDNA3.1-NKD1(human)-6×His

HZB811061_pEnCMV-TRIM7(human)-3×FLAG

HZB811062_pCMV6-AC-GFP-Tau(human)

HZB811063_pEF1-AMPK(human)-3×FLAG

HZB811064_pEnCMV-CCAR2(human)-3×HA

HZB811065_pAAV-NMNAT2((human)-FLAG

HZB811066_pCMVlacZ

HZB811067_pCas9-CAT

HZB811068_pEnCMV-Damt-1(elegans)-3×FLAG

HZB811069_pmirGLO-CPEB4

HZB811070_pCDNA3.1-KCNMB2-AS1(human)

HZB811071_pDNR-LIB-RPP40-2(human)

HZB811072_pOTB7-UQCRFS1-2(human)

HZB811073_pOTB7-ZNF324(human)

HZB811074_pOTB7-ZNF414-2(human)

HZB811075_pDNR-LIB-EPM2A(1同义突变)

HZB811076_pOTB7-TLE1-2(human)(1同义突变373-402缺失)

HZB811077_pOTB7-CYFIP2(human)(413-441缺失1点突变)

HZB811078_PL99

HZB811079_pOTB7-DDX23(human)

HZB811080_pDNR-LIB-IDI1-2(human)

HZB811081_pOTB7-EDEM1(human)(1同义突变)

HZB811082_pOTB7-AHCY-2(human)

HZB811083_pOTB7-ARPC5(human)

HZB811084_pCDNA3.1-NKD1(mut)-6×His(human)

HZB811085_pEnCMV-SEMA3A(human)-3×FLAG

HZB811086_pCDNA3.1-3×Myc-KRT14(human)

HZB811087_pEnCMV-IL4R(human)-3×FLAG

HZB811088_pEnCMV-RGS7(human)-3×FLAG

HZB811089_pMAD

HZB811090_pEF1-BECN1(human)-3×FLAG

HZB811091_pEF1-STK25(human)-3×FLAG

HZB811092_pEF1-HK2(human)-3×FLAG

HZB811093_pEF1-GTF2I(human)-3×FLAG(2同义突变)

HZB811094_pEnCMV-UPF1(human)-3×FLAG

HZB811095_pEnCMV-GRIN1-L135Q(rat)-3×FLAG

HZB811096_pMCS-Gaussia-Luc

HZB811097_pET28a-5DXW_A

HZB811098_pCMV-Gaussia-Luc

HZB811099_pEnCMV-SP1(mouse)-3×Myc

HZB811100_pBlueScriptR-PPFIA1

HZB811102_pCMV3-Spike(SARS-CoV)-FLAG

HZB811103_pEnCMV-Rac1(human)-3×FLAG

HZB811104_YE1-BE4max-NG

HZB811105_pEGFP-N1-3×Linker-E(SARS-CoV-2)

HZB811106_pEnCMV-CCAR2(human)-3×FLAG

HZB811107_pEnCMV-VGF(human)-3×FLAG

HZB811108_pEnCMV-HMGA2(human)-3×FLAG

HZB811109_pOTB7-RPL13A-2(human)

HZB811110_pOTB7-MXD4(human)

HZB811111_pLKO.1-DDit3-shRNA1

HZB811112_pOTB7-MRPL10(human)

HZB811113_pOTB7-FGR-2(human)

HZB811114_pOTB7-SCD(human)(1点突变)

HZB811115_pOTB7-IMPA2(human)(1-35截短1同义突变)

HZB811116_pOTB7-SSPN(human)

HZB811117_pOTB7-CORO6(human)

HZB811118_pOTB7-GPAT4-2(human)

HZB811119_pOTB7-ZBED2(human)

HZB811120_pOTB7-BRI3-2(human)(3同义突变)

HZB811121_pOTB7-HAPLN3-2(human)

HZB811122_pLKO.1-EGFR-shRNA1(pig)

HZB811123_pOTB7-BCL3(human)

HZB811124_pOTB7-ZBTB7A(human)(1点突变)

HZB811125_pOTB7-ZNF837(human)

HZB811126_pOTB7-ZNF282(human)

HZB811127_pOTB7-POP7(human)

HZB811128_pOTB7-FAM21A(human)(1点突变)

HZB811129_pET28a-SPAM1(human)(1同义突变)

HZB811130_pTD103aiiA(Cm)-NdeI

HZB811131_pCR-BluntII-TOPO-SEMA3A(human)(1同义突变)

HZB811132_pDNR-LIB-RHEX(human)

HZB811133_pLKO.1-EGFR-shRNA2(pig)

HZB811134_pOTB7-METRNL(1同义突变)(human)

HZB811135_pDNR-LIB-SPAG11A(2点突变)(human)

HZB811136_pDNR-LIB-LOC541473(多点缺失突变)(human)

HZB811137_pDNR-LIB-SEMG1(human)

HZB811138_pDNR-LIB-RPL26L1(human)

HZB811139_pDNR-LIB-FUT6(human)

HZB811140_pOTB7-DUSP5(3点突变)(human)

HZB811141_pOTB7-DIRAS2(human)

HZB811142_pDNR-LIB-MARC2(human)

HZB811143_pOTB7-CNTROB-2 (human)

HZB811144_pLKO.1-EGFR-shRNA3(pig)

HZB811145_pOTB7-CRLF1(1点突变)(human)

HZB811146_pOTB7-PSG1 (human)

HZB811147_pDNR-LIB-RPL23AP64(多点缺失突变)(human)

HZB811148_pDNR-LIB-IL17F(1点突变)(human)

HZB811149_pLV3-U6-SMS(mouse)-sgRNA3-Cas9-EGFP-Puro

HZB811150_pDNR-LIB-SLC2A2(human)

HZB811151_PiggyBac-CMV-HTR2B(human)-EF1a-Puro

HZB811152_pOTB7-TRIM41(human)

HZB811153_pOTB7-LOXL3(human)

HZB811154_pDNR-LIB-ZMAT2(human)

HZB811155_pENTR223-GLIPR1L1

HZB811156_pOTB7-DNAJB1(human)

HZB811157_pOTB7-GATD3B(human)

HZB811158_pDNR-LIB-RNF186-2(human)

HZB811159_pDNR-LIB-TDRD1(human)

HZB811160_pDNR-LIB-EXOSC5(1点突变)(human)

HZB811161_pDNR-LIB-NAALADL2(human)

HZB811162_pOTB7-AMFR(human)

HZB811163_pTagBFP-N-GRM2(mouse)

HZB811164_pCDNA3.1-Myc-SQSTM1(mouse)

HZB811165_pCDNA3.1-V5-HisB-MARK2(human)

HZB811166_pENTR223-GINS4

HZB811167_pEnCMV-FCGR3A(human)-3×FLAG

HZB811168_pCMV3-DsRed

HZB811169_pHR_Gal4UAS-HSV TK_IRES_mC_pGK_tBFP

HZB811170_pCDH-CMV-YAP1-S127D(human)-EF1-CopGFP-T2A-Puro

HZB811171_pCAMBIA1381-AtSAUR15-GUS

HZB811172_pOTB7-WT1-AS(多点缺失突变)(human)

HZB811173_pDNR-LIB-CSRP3(1同义突变)(human)

HZB811174_pDNR-LIB-TAGAP(human)

HZB811175_pOTB7-HRAS(human)

HZB811176_pOTB7-PAGE1(human)

HZB811177_pENTR223-DUSP19

HZB811178_pDNR-LIB-IGIP(human)

HZB811179_pOTB7-MRPS7(1点突变)(human)

HZB811180_pOTB7-GUF1(1点突变多1个碱基)(human)

HZB811181_pOTB7-FAM120B(human)(1同义突变)

HZB811182_pDNR-LIB-TMEM18-2(1同义突变)(human)

HZB811183_pOTB7-SNX1(1同义突变)(human)

HZB811184_pOTB7-FSD1(1同义突变)(human)

HZB811185_pOTB7-SRSF10-3(human)

HZB811186_pOTB7-ECHS1)(1点突变1同义突变)(human)

HZB811187_pOTB7-HPCA(human)

HZB811188_pENTR223-PRDX1

HZB811189_pOTB7-QPCTL(1同义突变)(human)

HZB811190_pOTB7-KCTD15(human)

HZB811191_pOTB7-ACP1-2(human)

HZB811192_pOTB7-UBR4(human)

HZB811193_pOTB7-NCLN-2(human)

HZB811194_pGL3-3×Mef2

HZB811195_pCDNA3.1-Spike(SARS-Cov-2)

HZB811196_pmEGFP-MATN3(human)-TEV-Twinstrep

HZB811197_pEnCMV-ZEB1(mouse)-3×Myc

HZB811198_pBC-Hygro-Cbh-MCS

HZB811199_pENTR223-PRAF2

HZB811200_pET32a-CUP1-2(yeast)