凯杰 QIAGEN 208054 QuantiNova SYBR Green PCR Kit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova SYBR Green PCR Master Mix, 500 µl QuantiNova Yellow Template Dilution Buffer, 1 ml QN ROX Reference Dye, 1.9 ml Water
通过基于SYBR Green的qPCR获得好的结果
- 通过新型热启动机制获得良好的特异性
- 通过内置颜色指示剂实现准确的反应构建
- 精确、灵敏的检测低至单拷贝的靶序列
- 采用超快速PCR技术，提高样本通量和反应速度
- 与QuantiTect Primer Assay实现无缝对接
QuantiNova SYBR Green PCR Kit具有高特异性、敏感度、反应速度和处理安全性，为SYBR Green-based qPCR技术树立了全新标准。创新的热启动技术显著增加了real-time PCR特异性，通过使用优化的试剂，能够在任何real-time PCR仪上从cDNA或gDNA——甚至是数量低至1个拷贝的靶标——获得精确的定量检测结果。此外，内置的颜色指示剂帮助确保加样的准确性。
Product Details
Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.
Avoid the deleterious effects of mispriming at lower temperatures with the QuantiNova Probe PCR Kit (probe qPCR kit). Based on a novel, antibody-mediated, hot-start mechanism, the kit enhances the specificity and efficiency of probe-based real-time PCR to provide accurate, singleplex or duplex, cDNA or gDNA analysis on various real-time PCR cyclers. The added convenience of extreme stability for up to 100 hours at room temperature, without a need for any cooling agent, makes it ideal for handling of high-throughput samples and automated workflows. An in-built tracking system for visual identification of correct pipetting gives you absolute peace of mind while extremely sensitive detection of even low target amounts ensures reliable qPCR results every time.
QuantiNova Multiplex PCR Kits (multiplex qPCR kits) enable fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote ultrafast multiplex real-time PCR within 1 hour. The combination of a unique hot start and PCR buffer system in the ready-to-use 4x master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization, also providing automated reaction set up options at room temperature. An in-built tracking system for visual identification of correct pipetting helps to prevent human errors, and the combination with QuantiNova Reverse Transcription Kit for cDNA synthesis allows inclusion of QuantiNova Internal Control RNA to monitor successful reverse transcription and qPCR.
Performance
Want to try the QuantiNova SYBR Green PCR Kit for the first time? Request a quote for a trial kit.
Want to try the QuantiNova Probe PCR Kit for the first time? Request a quote for a trial kit.
Want to try QuantiNova Multiplex PCR Kits for the first time? Request a quote for a trial kit
Applications
The QuantiNova SYBR Green PCR Kit can be used for SYBR Green-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, LIFE Technologies, Roche, and Agilent, as well as the Rotor-Gene Q.
QuantiNova Probe PCR Kits can be used for probe-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent.
QuantiNova Multiplex PCR Kits can be used for multiplex gene expression analysis of cDNA or gDNA targets on any real-time cycler. To fully exploit the capability of multiplex benefits, we recommend instruments providing up to 5plex capacity, such as the Rotor-Gene Q.
