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The pCDH-MSCV-MCS-EF1α-GFP lentivector uses the MSCV promoter for high expression in hematopoietic or stem cells and includes a GFP reporter
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-MSCV-MCS-EF1α-GFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the MSCV promoter, which provides strong expression in hematopoietic and stem cells. This lentivector also enables selection for transductants using a GFP reporter.
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
Product Documentation
- User Manual: HIV-based cDNA Expression Lentivectors
Citations
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- Vasjari, L, et al. (2018) Ras signals principally via Erk in G1 but cooperates with PI3K/Akt for Cyclin D induction and S-phase entry. Cell Cycle. 2018 Dec 18;. PM ID: 30560710
- Verhagen, HJMP, et al. (2018) IGFBP7 Induces Differentiation and Loss of Survival of Human Acute Myeloid Leukemia Stem Cells without Affecting Normal Hematopoiesis. Cell Rep. 2018 Dec 11; 25(11):3021-3035.e5. PM ID: 30540936
- Lee, PC, et al. (2018) Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell. 2018 Dec 10; 34(6):954-969.e4. PM ID: 30537515
- Schoderboeck, L, et al. (2018) Genetic targeting and chemogenetic inhibition of new born neurons. Hum Gene Ther Methods. 2018 Dec 7;. PM ID: 30526082
- Gianni, M, et al. (2018) S100A3 a partner protein regulating the stability/activity of RARα and PML-RARα in cellular models of breast/lung cancer and acute myeloid leukemia. Oncogene. 2018 Dec 7;. PM ID: 30532072
- Huang, Z, et al. (2018) Hsa-miRNA-29a protects against high glucose-induced damage in human umbilical vein endothelial cells. J. Cell. Biochem.. 2018 Dec 5;. PM ID: 30520097
- Shen, CY, et al. (2018) The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells. Oncogenesis. 2018 Dec 5; 7(12):95. PM ID: 30518871
- Briard, B, et al. (2018) Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection. Nat Microbiol. 2018 Dec 3;. PM ID: 30510167
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