Description
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
• Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold® DNA Polymerase, LD to offer greater sensitivity than classic SYBR® Green PCR master mix
• Power SYBR® Green PCR Master Mix contains a blend of dTTP/dUTP that is compatible with AmpErase® UNG, to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
• Power SYBR® Green reagent-based PCR chemistry easily replaces the existing SYBR® Green PCR Master Mix in Applied Biosystems® protocols using the same setup and thermal cycling conditions
Alternative product
Try PowerUP SYBR Green Master Mix,our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.
Specifications
| Detection Method: | SYBR |
|---|---|
| For Use With (Equipment): | 7300 System,7500 System,7700 System,7900HT Fast System,QuantStudio™ 12k Flex,QuantStudio™ 3,QuantStudio™ 5,QuantStudio™ 6 Flex,QuantStudio™ 7,StepOne™, Standard Mode,StepOnePlus™, Standard Mode,ViiA™ 7 System |
| Label or Dye: | SYBR® Green |
| PCR Method: | qPCR |
| Passive Reference Dye: | ROX (Pre-mixed) |
| Polymerase: | AmpliTaq Gold DNA Polymerase |
| Primer-Probe Compatibility: | Unlabeled Oligos |
| Product Line: | SYBR® |
| Product Size: | 1 x 5 mL |
| Purity or Quality Grade: | LD (Low DNA) |
| Reaction Speed: | Standard |
| Sample Type (General): | DNA |
| Sample Type (Specific): | DNA (Genomic),cDNA |
| Shipping Condition: | Wet Ice |
Contents & storage
The 2X mix contains SYBR® Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 5 mL, sufficient for 200 reactions of 50 µl each.
Store at -20°C.
For Research Use Only. Not for use in diagnostic procedures.
