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2×Taq PCR Master Mix(Green)

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  • 28.89/

北京百瑞极生物科技有限公司
010-82422342
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  • Brand  Biorigin
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Last Updated 2025-08-12 20:11

2×Taq PCR Master Mix(Green)

产品内容

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储存条件 -20℃保存,有效期一年。

产品介绍 

本产品包含 Taq DNA Polymerase、dNTP、指示染料以及优化的缓冲体系,浓度为 2×。产品使用便捷,使用时只需加入模板、引物和水,使反应体系浓度为 1×即可进行 PCR 反应。

该 PCR Mix 最长可以扩增 5kb DNA 片段,具有良好的扩增特异性和模板兼容性,由于PCR mix 中含有染料,扩增产物可直接点样电泳。

产品特点 

操作简便,可减少人为误差; 

扩增效率高,稳定性好; 

适合复杂模板和较长片段的扩增。

使用方法 

1. 常规 PCR 反应体系

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注:不同模板最佳反应浓度有所不同,以 50 μL 体系为例:模板为基因组 DNA 时,一般应在 10~400 ng;模板为质粒或病毒 DNA,一般应在 10 pg~20 ng。

2. 反应程序

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注意事项 

使用时彻底化冻、混匀;PCR 反应体系请在冰上进行配制,之后再置于PCR 仪上进行反应,减少非特异性扩增。

Company Name 北京百瑞极生物科技有限公司
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