小鼠肺微血管内皮细胞系、永生化小鼠肺微血管内皮细胞、小鼠肺血

永生化小鼠肺微血管内皮细胞简介：

产品介绍
产品名称：小鼠肺微血管内皮细胞系、永生化小鼠肺微血管内皮细胞
产品描述：脑微血管内皮细胞是血脑屏障的主要组成成分，能够限制可溶性物质和细胞等从血液进入大脑。大脑微血管内皮细胞与外周内皮细胞相比具有一些相同特性。1）脑微血管内皮细胞存在许多细胞间紧密连接，产生很高的跨内皮阻抗，延迟细胞旁的通量；2）脑微血管内皮细胞缺乏内皮细胞的窗孔结构，其液相物质胞饮水平较低；3）脑微血管内皮细胞具有不对称定位酶和载体介导转运系统，从而产生 “两极分化”的表现型。 与外周内皮细胞相同，大脑微血管内皮细胞表面表达细胞粘附分子，调控白细胞进入大脑。由于微血管内皮细胞的器官特异性，内皮细胞通常取源于疾病研究的相关组织。
产品货号：IM2024
产品类型: 永生化细胞
传代能力: 可以30代传代
产品形态: 上皮样
培养基：永生化小鼠肺微血管内皮细胞完全培养基
支原体：阴性
产品培养条件：37℃，5%CO₂
发货方式：T25瓶子常温发货
货期：3-5天货期  

vWF抗体免疫荧光染色鉴定

[Role of RAGE in lipopolysaccharide-induced cytoskeletal changes in mouse pulmonary microvascular endothelial cells].

OBJECTIVE: To investigate lipopolysaccharide (LPS)-induced changes of cytoskeletal filamentous actin in primary isolated pulmonary microvascular endothelial cells (PMVECs) from wild-type and RAGE knock-out mouse. METHODS: The lungs of wild-type and RAGE knock-out mice were digested with collagenase type I to obtain endothelial cells purified by anti-CD31-coupled magnetic beads. The PMVEC identified by factor VIII labeling were stimulated with LPS at different concentrations and the changes of filamentous actin were observed by confocal microscopy. RESULTS: The cultured primary cells showed typical endothelial cell phenotype as examined with factor VIII labeling. LPS stimulation caused rearrangement of the cytoskeletal filament F-actin in wild-type mouse PMVECs with stress fiber formation, but such changes were not obvious in RAGE knock-out mouse PMVECs. CONCLUSION: Mouse PMVECs of a high purity can be obtained by immune magnetic beads. RAGE is involved in LPS-induced destruction of mouse PMVEC cytoskeletons.