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Features of the TA Cloning® Kit with pCR™2.1 vector:
• Fast & convenient—15-minute, room-temperature ligation
• Efficient—blue/white screening and >80% clones with correct insert
• Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—eliminates any enzymatic modifications of the PCR product
• Streamlined—does not require the use of PCR primers that contain restriction sites
The pCR™2.1 vector provides:
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter for in vitro RNA transcription and sequencing
• A versatile polylinker with flanking EcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing
How TA Cloning® Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products. The linearized vector supplied in this kit has single 3' deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.
Kit Configurations
The TA Cloning® Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot® INVF' Chemically Competent E. coli (K2000-01 and K2000-40), with One Shot® TOP10F' Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot® TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes.
内容及储存
TA Cloning® kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
