DO/-Ura

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  • 90.28/

北京兰博利德商贸有限公司
15711203280 01060608020
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  • Brand  兰博利德(LABLEAD)货号Y2013102
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The information on this page regarding the "DO/-Ura" product is provided by 北京兰博利德商贸有限公司. If you would like to know more about the price, model, and manufacturer of "DO/-Ura", please contact the supplier or leave a message.
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Brand 兰博利德(LABLEAD)货号Y2013102
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Last Updated 2025-08-12 20:15

DO/-Ura

DO/-Ura

保存:室温保存

产品简介:

DO Supplement(Dropout Supplement),是由必需氨基酸和两种核苷酸组成的混合物,用于制备酵母SD 培养基(由 YNB、葡萄糖、氨基酸和核苷酸分子组成)。任何一种 DO Supplement 都缺失一种或多种成分,借此来筛选可以表达该成分的菌株或用来激活相关报告基因。例如 DO Supplement-His 含有除了组*酸外的所有必需氨基酸和腺嘌呤,尿*啶,可以用来筛选含有表达组*酸合成基因的质粒的酵母阳性菌株;可用于 pLexA 等质粒的转化筛选和组*酸报告基因的激活实验;或用于酵母互作和酵母蛋白表达,离子耐受,功能互补及突变体鉴定、分类等实验。

使用方法:(以配制 SD Agar 培养基为例)

按照标签称取 DO Supplement(约 1.3g)加入 1L 去离子水中,然后分别加入 6.7g YNB(PM2070), 20g 葡萄糖,搅拌溶解,调节 pH 至 5.8,再加入 20g 琼脂粉。121℃高压灭菌 15min,冷却到 50-60℃,把培养基倒入平板中,室温使其凝固后使用,或封口膜封好后倒置保存在 4℃冰箱。

 

产品货号及规格:

分类

名称

货号

规格

Single Dropout Supplement

(SDO/单缺)

DO/-Trp

Y2013101

20g

DO/-Ura

Y2013102

20g

DO/-His

Y2013103

20g

DO/-Leu

Y2013104

20g

Single Dropout Supplement

(DDO/二缺)

DO/-His-Ura

Y2013201

20g

DO/-His-Trp

Y2013202

20g

DO/-Trp-Ura

Y2013206

20g

DO/-Leu-Ura

Y2013207

20g

DO/-Met-Trp

Y2013208

20g

DO/-Leu-Trp

Y2013220

20g

DO/-His-Leu

Y2013221

20g

DO/-Ade-Trp

Y2013223

20g

Triple Dropout Supplement

(TDO/三缺)

DO/-Ade-His-Trp

Y2013301

20g

DO/-Leu-Trp-Ura

Y2013302

20g

DO/-Leu-Met-Trp

Y2013309

20g

DO/-His-Leu-Trp

Y2013323

20g

DO/-His-Leu-Ura

Y2013324

20g

DO/-His-Trp-Ura

Y2013325

20g

Quadruple Dropout Supplement

(QDO/四缺)

DO/-His-Leu-Met-Trp

Y2013401

20g

DO/-Ade-His-Leu-Trp

Y2013406

20g

DO/-His-Leu-Trp-Ura

Y2013407

20g

DO Supplement

氨基酸混合物

Y0005

10g

Company Name 北京兰博利德商贸有限公司
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Medicine Platform
 
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