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Hiseq2500二代测序仪

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北京拓普塞斯生物技术有限公司
13121536697
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Last Updated 2025-08-08 21:27

Hiseq2500二代测序仪

技术指标: 1高数据通量:每次仪器运行单端读取至少可生成300G碱基数据,双端读取至少可生成600G碱基数据;在快速运行模式下27h内产生120G数据 2 实验时间:单端读取4天,双端读取8天(根据测序长度略有变化);                   快速模式27h 3运行成本:从样品制备到数据读取,每测量100万个碱基对所需成本不高于0.05美元 4数据读长:单端读取序列,读长大于100个碱基; 5双端读取序列:高通量模式读长2x100个碱基;快速模式读长2x150个碱基 6样品用量:DNA样本需要0.1-1微克gDNA或cDNA 7读取精度:原始数据准确性≥98.5%,3倍覆盖拼接序列准确性≥99.99% 8可精确读取≥10个的连续单个重复碱基(如AAAAAAAAAA),准确性≥98.5% 9不同样品并行性:每次仪器运行可平行分析8个非混合的独立样品 10 可开展ChiP-seq、小RNA发现的定量、未知物种测序、转录组研究、甲基化研究试剂盒,宏基因组研究、重测序、目标区域重测序。
   
 
   
使用范围: Hi-seq2500主要用途: 1.未知物种基因组的从头测序 2.已经测序物种的基因组重新测序 3.基因组尺度转录组学分析(Transcriptomics) 4.基因表达谱及基因调控研究 5.全基因组小分子RNA(miRNA)分析 6.染色质免疫互沉淀研究(ChIP) 7.多重单核苷酸多型性(SNP)分析 8.甲基化研究 9. 其他表观遗传学研究如磷酸化修饰 10.蛋白-DNA相互作用研究 11. 染色体重排研究(缺失,插入等等)
Company Name 北京拓普塞斯生物技术有限公司
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