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Bio-rad伯乐PROTEAN II xi 和XL大型垂直

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北京赛百奥科技有限公司
13601128645 010-51282213
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  • Brand  BIO-RAD
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  • Main Business:核转仪,纯水仪,凝胶成像系统,美国伯乐电泳槽,电泳仪,紫外分析仪,PCR仪
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Last Updated 2025-08-08 22:31

Bio-rad伯乐PROTEAN II xi 和XL大型垂直

名称:PROTEAN II xi 和XL 大型垂直电泳槽
产品简介:

PROTEAN II xi 电泳槽和PROTEAN II XL 宽式电泳槽功能灵活,可用于多种分离,包括SDS-PAGE、第 一和第二向双向电泳、非变性、制备、梯度和高分辨 率核酸琼脂糖电泳。单向电泳( 1-D) 可使用 PROTEAN II xi 电泳槽进行16 x 16 cm 和16 x 20 cm 凝胶电泳。双向电泳(2-D),使用PROTEAN II XL 电 泳槽电泳可容纳17和18 cm IPG 胶条的第二向凝 胶(约18.5 x 20 cm)。

品牌:伯乐bio-rad

 

PROTEAN II  xi 和XL 大型垂直电泳槽

 

大型垂直电泳槽-1.jpg

 

【介绍】

PROTEAN II xi 系统组件

  1. 电泳槽及盖(Tank and lid)
  2. 中央制冷芯(Central cooling      core)
  3. 托架(黑色) (Latch (black)
  4. 灌胶架(Casting stand)
  5. 三明治夹(Sandwich clamps)
  6. 胶板对位卡(Alignment card)
  7. 电泳梳(Combs)

 

PROTEAN II xi 电泳槽和PROTEAN II XL 宽式电泳槽功能灵活,可用于多种分离,包括SDS-PAGE、第 一和第二向双向电泳、非变性、制备、梯度和高分辨 率核酸琼脂糖电泳。单向电泳( 1-D) 可使用 PROTEAN II xi 电泳槽进行16 x 16 cm 和16 x 20 cm 凝胶电泳。双向电泳(2-D),使用PROTEAN II XL 电 泳槽电泳可容纳17和18 cm IPG 胶条的第二向凝 胶(约18.5 x 20 cm)。

※ 灌胶架上专利的单螺纹夹*可在全长玻板上均 匀施加压力,防漏密封设计,无需封脂类或 琼脂糖

※ 中央制冷芯可外接水循环或一个冷却再循环 水浴,或灌装冷却剂作为散热器使用。只需 要1.5 L 缓冲液就可以保证凝胶结果不会出现 “微笑”现象。

※ 可选择两种长度的凝胶:16 或20 cm,根据 所选的凝胶长度选择相应的玻板、封边垫条和三 明治夹(所有其他组件均要与相应的凝胶长度匹配)

※ 使用495 型梯度生成仪、PROTEAN II 多 板凝胶灌胶器和合适的对数活塞可灌制多种梯度胶;容量为7-50 ml 的475 型梯度生成系统与PROTEAN II 板式凝胶灌 胶架是灌制单一梯度凝胶的理想选择

※ 使用带有凹口的玻板(notched plates),可同时进行4 块16 x 16 cm 或16 x 20 cm 手灌胶电泳,可增加一次电泳的样品数(不推荐用于双向电泳)

※ 可选择磨砂玻板(frosted glass)进行高分辨率的垂直琼脂糖核酸电泳;电泳 梳转换螺纹夹(comb conversion screws) 可把任何PROTEAN II xi 电泳梳从标 准的25 mm 孔深转变为10 mm 孔深,并方便的将电泳梳从易碎琼脂糖凝胶中 取出

* 美国专利4,574,040

 

xi1.png

选择带有凹口的内玻板,增 加封边垫条使PROTEAN II xi 电泳槽能进行4块凝胶电泳。

 

简单的装配流程

 

1. 单螺纹凝胶夹装配凝胶三明治。

xi2.png

2. 用制胶架的对位槽和凝胶对位对齐凝胶三明治夹;

xi3.png

3. 完成制胶后将凝胶三明治按扣到冷却芯上。

xi4.png

4. 样品上样于凝胶表面。

xi5.png

 

5. 若要进行第一向IEF,使用PROTEAN II xi 2-D 电泳槽和自带的管式凝胶适配器,可同时电泳

xi6.png

6. 第一向管式凝胶或ReadyStrip IPG 胶

xi7.png

 

 

应用于双向电泳的PROTEAN II IPG 转换组合套件

PROTEAN II IPG 转换组合套件可把经典的PROTEAN II xi 电泳槽和多板电泳槽规格扩大为XL 格式,以适用于 IPG 胶条的第二向电泳

※ 凝胶宽度增加到18.5 cm,适合17 cm 及18 cm ReadyStrip IPG 胶条

※ 转换组合套件包括凝胶夹(gel clamps)、封边垫条(Spacers)、2-D电泳梳(2-D combs)、密封衬垫(gaskets)和玻板(glass plates),适合1.0 mm、1.5 mm或2.0 mm厚度的凝胶

※ PROTEAN II XL 电泳槽包括PROTEAN II IPG 转换组合套件

 

【技术指标】

 

PROTEAN II xi
  (16 cm)

PROTEAN II xi
  (20 cm)

PROTEAN II XL
  (20 cm)

凝胶大小(W x L)

16 x 16 cm  

16 x 20 cm  

18.5 x 20 cm

玻板规格(W x L)

     

  内板

16 x 20 cm  

20 x 20 cm  

20 x 20 cm

  外板

18.3 x 20 cm  

22.3 x 20 cm  

22.3 x 20 cm

封边垫条长度

18.3 cm

22.3 cm

22.3 cm

典型上层缓冲液容积

350 ml

350 ml

350 ml

典型下层缓冲液容积

1.8 L

1.2 L

1.2 L

典型SDS-PAGE电泳时间*

     

  无冷却

4 小时

5 小时

5 小时

  带冷却

2.5 小时

3.5 小时

3.5 小时

 * 与电泳相关的电压和电流设置。

 

【订货信息】

目录

描述

PROTEAN II xi Cells

165-1801

PROTEAN II xi Cell, 16 cm,   without spacers and combs

165-1802

PROTEAN II xi Cell, 16 cm, 1.5 mm spacers (4), 15-well   combs (2)

165-1803

PROTEAN II xi Cell, 16 cm, 1.0 mm spacers (4), 15-well combs (2)

165-1804

PROTEAN II xi Cell, 16 cm, 0.75 mm spacers (4), 15-well   combs (2)

165-1811

PROTEAN II xi Cell, 20 cm,   without spacers and combs

165-1812

PROTEAN II xi Cell, 20 cm, 1.5 mm spacers (4), 15-well   combs (2)

165-1813

PROTEAN II xi Cell, 20 cm, 1.0 mm spacers (4), 15-well combs (2)

165-1814

PROTEAN II xi Cell, 20 cm, 0.75 mm spacers (4), 15-well   combs (2)

165-1815

PROTEAN II xi Cell 2-D Conversion Kit, includes 2 tube gel adaptors, 24 glass tubes (1.5 mm ID, 180 mm length), gaskets,   grommets, stoppers

165-1834

PROTEAN II xi Basic Unit With Casting Stand, includes central cooling core, lower buffer chamber,   lid with cables, leveling bubble; combine with an IPG conversion kit for a   complete 18.5 cm   wide-format system

PROTEAN II XL Cells Compatible With IPG Strips

165-3188

PROTEAN II XL Cell, wide format, 1.0 mm,   includes PROTEAN II xi basic unit (catalog #165-1834) and 1.0 mm IPG conversion kit (catalog   #165-3183)

165-3189

PROTEAN II XL Cell, wide format, 1.5 mm,   includes PROTEAN II xi basic unit (catalog #165-1834) and 1.5 mm IPG conversion kit   (catalog #165-3186)

165-3190

PROTEAN II XL Cell, wide format, 2.0 mm,   includes PROTEAN II xi basic unit (catalog #165-1834) and 2.0 mm IPG conversion kit (catalog   #165-3184)

IPG Conversion Kits

165-3183

PROTEAN II xi Cell IPG Conversion Kit, 1.0 mm,   18.5 x 20 cm,   includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG 2-D combs,   2 IPG central cooling core gaskets, 2 casting stand gaskets, alignment card

165-3184

PROTEAN II xi Cell IPG Conversion Kit, 2.0 mm,   18.5 x 20 cm,   includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG 2-D combs,   2 IPG central cooling core gaskets, 2 casting stand gaskets, alignment card

165-3186

PROTEAN II xi Cell IPG Conversion Kit, 1.5 mm,   18.5 x 20 cm,   includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG 2-D combs,   2 IPG central cooling core gaskets, 2 casting stand gaskets, alignment card

Company Name 北京赛百奥科技有限公司
Location
Main Products or Services 核转仪,纯水仪,凝胶成像系统,美国伯乐电泳槽,电泳仪,紫外分析仪,PCR仪
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