Agilent Stratagene Mx3005P,Mx3

Mx4000™ Multiplex Quantitative PCR System

New System for Advanced QPCR

Stratagene delivers a new standard in QPCR with the Mx4000™ multiplex quantitative PCR system. This new system offers superior performance, more flexibility, and reasonable pricing.

• Exceptional reliability and reproductivity
• Real-time plots while your PCR progresses
• Superior dye discrimination
• Better temperature uniformity

MX4000 Real-time quantitative PCR (QPCR) allows researchers to quickly and easily quantify nucleic acids for studying gene expression, mutational analysis, disease state, and gene dosage. QPCR measures PCR product accumulation during the exponential phase of the reaction and before amplification becomes vulnerable to limiting reagents and cycling variability. Fluorescent QPCR data provides accurate information on initial starting copy number.

Using QPCR, amplification and detection are combined in a single step and in a single closed tube. This eliminates the need for numerous post-PCR manual steps, and reduces the possibility of introducing variability or laboratory contamination.

Flexible System with More Features

The Mx4000 system detects multiple fluorescent PCR chemistries and combines the capabilities of a microplate fluorescence reader with a PCR thermal cycler into a single real-time detection system. The Mx analyzer integrates precision thermal control with a multi-wavelength detection system. Applications include allelic discrimination, plate reads, melting curves, and quantification of single or multiplexed targets.

Superior Dye Discrimination

Extended Spectrum

The Mx4000 multiplex quantitative PCR system provides a detection range of 350 nm to 830 nm, allowing greater flexibility of fluorophore choice, providing high sensitivity and excellent signal-to-noise ratio. The Mx4000 system’s light source generates an extended excitation range from 350 to 750 nm. This allows choosing of fluorophores with little or no spectral overlap, producing clean, delineated signals for superior multiplexing. Each of the four scanning fiber-optic heads independently excites and detects dyes, reading up to four different dyes in a single tube.

Optimized Filters

Optimized interference filters precisely match the excitation and emission wavelengths for each fluorophore to block out unwanted cross-talk from spectrally adjacent fluorophores. Researchers can choose from FAM, TET, HEX/JOE/VIC, TAMRA, Texas Red/ROX, Cy5 and Cy3 filter sets. Custom filter sets are also available for other fluorophores.asefont>

asefont>Intuitive, Easy-to-Use Software

asefont>Your options are increased with new features such as real-time amplification plots that can be viewed as your PCR progresses. It makes many applications easier than ever before, such as real-time and plate-read allelic discrimination, real-time QPCR, qualitative detection, and melting curve profiles.

asefont>View Real-Time Plots while PCR Progresses

asefont>Many features are included, including real-time amplification plots as the run progresses. This allows you to determine at a glance how an experiment is running at any time during thermal cycling, rather than waiting until the end of the run. You can choose to abort a run if a problem develops in a reaction, or stop the experiment and save the data as soon as the desired information is generated.

asefont>More Options

asefont>The ability to make allelic discrimination calls by endpoint fluorescence values, as well as by threshold cycle is also featured. Data can be viewed in many forms including amplification plots, scatter plots, sample value screens for the entire plate for all dyes, fluorescence intensity screen, final call results, melting curves, annealing range, and text reports.

asefont>A time-saving protocol allows you to select a subset of the 96 wells for independent analysis, allowing you to perform multiple sub-experiments on a single plate.

asefont>Better Temperature Uniformity for More Precise PCR

asefont>The thermal system delivers unsurpassed temperature control to the sample, using patent-pending solid-state heating and cooling technology. Superior thermal ramp rates, unparalleled thermal accuracy, and the best temperature uniformity in a 96-well plate format are performance benefits of this advanced system. The extremely low thermal mass of the sample thermal control block promotes rapid changes in temperature, so your experiments are completed in less time. A typical 96-sample quantitative PCR thermal profile of 40 cycles can be completed in less than 90 minutes.

asefont>Better Communication Between Instrument and PC

asefont>The software establishes robust communication between the instrument and the attached personal computer. Even in the event of a power loss to the PC or communications error, data collection continues. When communication is restored, the data from the run is transferred from the instrument’s embedded software to the software on the PC - ensuring that the experiment will be completed and your data successfully saved.

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