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大鼠维生素D3(VD3)ELISA试剂盒

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上海亿修生物科技有限公司
17317157434 00000000
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  • Main Business:Elisa试剂盒,超声波破碎仪
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Last Updated 2025-08-08 23:34

大鼠维生素D3(VD3)ELISA试剂盒

ELISA试剂盒

ELISA试剂盒组成:

 

 

ELISA试剂盒检测原理:

双抗体夹心法将特异性抗体(捕获抗体)与固相载体连接,形成固相抗体。洗涤除去未结合的抗体及杂质。加入待检样品,保温孵育。样品中的特异性抗原与固相抗体结合,形成固相抗原抗体复合物。洗涤除去其他未结合物质。加酶标抗体(检测抗体)保温孵育。固相免疫复合物上的抗原与酶标抗体结合。彻底洗涤未结合的酶标抗体。加底物反应。

操作程序:

 

上海亿修生物ELISA试剂盒 操作注意事项:
1 试剂应按标签要求储存,使用前恢复到室温。稀稀过后的标准品应丢弃,不可保存;

2 实验中不用的板条应立即放回包装袋中,密封保存,以免变质。
3 不用的其它试剂应包装好或盖好,不同批号的试剂不要混用。保质前使用。

4 使用一次性的吸头以免交叉污染,吸取终止液和底物A、B波时,避免使用带金属部分的加样器。

5使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

上海亿修生物试剂盒优势方面:

Company Name 上海亿修生物科技有限公司
Location
Business Scope Elisa试剂盒,超声波破碎仪
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