For Sale Agilent Advanced Analytical Fragment Analyzer 5300

Internal part number SM100786-5300

• Fully Functional
• Serviced & in Stock
• Good cosmetic condition
• Technical sales consultation available
• 60 DAYS INTERNATIONAL WARRANTY
• Includes remote instrument setup and training session 

Made by Advanced Analytical, now branded by Agilent as 5300

Here is what you will receive:

• Advanced Analytical Fragment Analyzer
• (4) Marker/sample PCR microplate (semi or non-skirted) holders
• (2) Deep-well microplate holders
• (2) 250ml bottles
• 50ml bottle
• 500ml waste bottle
• Syringe for valve flush
• Computer running Fragment Analyzer ProSize data analysis software
• Fragment Analyzer controller software
• Communication and power cables

Please note: Capillary Array is a consumable and has one year or 1000 injection lifespan, a new one will need to be purchased from Agilent.

Advanced Analytical 5300 Fragment Analyzer Configuration:

• Instrument Applications: NGS libraries, cell-free DNA, genomic DNA, large fragment DNA, PCR fragments, total RNA and IVT RNA
• Analysis Method: fluorescence-based capillary electrophoresis using intercalating dye
• Capillary Array: compatible with 48 and 96 capillary arrays
• Minimum Sample Volume: 25 uL of liquid for injection; as little as 2.5 uL of actual sample required
• Sample Capacity: 3 sample trays, each holding 1x 96 well PCR microplate
• Compatible Microplates: 96 well semi or non-skirted PCR plates for markers and samples, 96 deep-well plates for buffer and waste
• Number of Samples per Run: 48 or 96 (Depending on the installed Array)
• Variable Throughput: up to 240 samples, medium throughput system
• High Sensitivity: sensitivities down to 5 pg/uL for a single fragment, cycle time or PCR reagent
• Resolution and Sizing Accuracy: resolve small differences between fragments, as low as 3 bp for fragments under 300 bp size
• Power requirements: 100-240 VAC; 50/60 Hz
• Dimensions (H x W x D): 61 cm x 38 cm x 51 cm (24 x 14 x 20 in)
• Weight: 38.5 kg (85 lbs)

Customization/Options: N/A

Testing performed by our in-house service team:

• We check the power supply to ensure it provides stable output.
• We test communication with the software to verify seamless interaction and data exchange.
• We test the initialization and homing processes to guarantee accurate and reliable setup.
• We check the pump operation to ensure consistent and reliable fluid movement.
• We test the plate movement to validate precise and smooth sample handling.
• We thoroughly test the prime function of all fluids to ensure the system is clean and functions as designed.
• We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
• We test the high voltage system from minimum to maximum levels to verify consistent and safe operation.
• We perform an Optical Alignment on a Capillary Array, verifying the camera and illumination are operating fully.

Instrument Overview: 

• The Agilent Advanced Analytical 5300 Fragment Analyzer is a versatile instrument for nucleic acid analysis.
• It offers high-resolution separation and quantification of DNA, RNA, and PCR fragments, enabling accurate characterization of sample size distribution.
• With its automated workflow and intuitive software interface, it streamlines experimental processes and data analysis.
• The analyzer's sensitivity and precision make it suitable for a wide range of applications, including next-generation sequencing library preparation, genotyping, and quality control.
• Designed for flexibility, the Fragment Analyzer accommodates various sample types and sizes, providing researchers with comprehensive insights into nucleic acid samples.
• Overall, the 5300 Fragment Analyzer is a valuable tool for molecular biology research, diagnostics, and biotechnology applications.

Instrument Application: 

• NGS libraries
• Cell-free DNA
• Genomic DNA
• Large fragment DNA
• PCR fragments
• Total RNA and IVT RNA

The Key Features:

• High-precision DNA/RNA analysis
• High-resolution separation
• Medium- to high-throughput separation
• User-friendly interface
• Reduced sample analysis time
• Broad assay portfolio
• Controlled sample handling
• Seamless switching between applications

Why do so many customers choose to order from us?

Our Advantage

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.  see questions section below  
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

ME Medium Crate
350#

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper.  If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.  

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries. 

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.